Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
货号：
2707S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 2707S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total Zap-70 protein. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). |
| 存放说明 | 4C |
参考图片Two-color flow cytometric analysis of a mixed population of T and B cells (Jurkat and Ramos, respectively) using Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) and a CD3 antibody. CD3 negative B cells have little or no Zap-70 staining, while CD3 positive T cells stain brightly for Zap-70 protein.双光流式细胞仪分析T细胞和B细胞的混合液(分别为Jurkat 和 Ramos)。所用抗体为Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) 和CD3 antibody。CD3 阴性 B 细胞具有很少或者没有Zap-70 蛋白染色, CD3 阳性的T 细胞能够有很强的Zap-70蛋白信号。 |
Flow cytometric analysis of Ramos B (blue) and Jurkat T (green) cells using Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate).流式细胞仪分析Ramos B (蓝色) 和Jurkat T (绿色)细胞，所用的抗体为Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate)。 |
