| 货号 | 3310S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells.该CST公司生产的抗体与Alexa Fluor®488荧光染料偶联并能对人的细胞进行流式细胞术和免疫荧光检测。 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 目标/特异性 | c-Kit (Ab81) Mouse mAb detects endogenous levels of total c-Kit protein. It does not cross-react with other receptor tyrosine kinase family members. |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7). |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat (blue) and H526 (green) cells using c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate).用c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate)对Jurkat (蓝色)和 H526 (绿色)细胞进行流式细胞术检测。 | |
Confocal immunofluorescent analysis of H526 cells using c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用c-Kit (Ab81) Mouse mAb (Alexa Fluor® 488 Conjugate)（绿色）对H526细胞进行免疫共聚焦检测。蓝色 = DRAQ5® #4084进行的DNA染色 |
