| 货号 | 4853S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated COX IV (3E11) Rabbit mAb #4850. |
| 反应种属 | Human/Rat/Monkey/ZebrafishBovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total COX IV protein. |
| 使用方法 | F(1:50) IF-IC (1:100) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Deficiency in COX activity has been correlated with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control. |
| 存放说明 | 4C |
Flow cytometric analysis of NIH/3T3 (blue) and Jurkat cells (green) using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate). 流式细胞式检测NIH/3T3 (蓝) and Jurkat cells (绿)，所用抗体为COX IV (3E11) Rabbit mAb兔单抗 (Alexa Fluor® 488 Conjugate). | |
Confocal immunofluorescent analysis of SKOV3 cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). 激光共聚焦免疫荧光检测SKOV3细胞，所用抗体为COX IV (3E11) Rabbit mAb兔单抗 (Alexa Fluor® 488 Conjugate) (绿) and β-Tubulin (9F3) Rabbit mAb兔单抗 (Alexa Fluor® 555 Conjugate) #2116 (红)。 |
