| 货号 | 5198S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated EpCAM (VU1D9) Mouse mAb #2929. |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | IF-IC/F |
| 目标/特异性 | EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total EpCAM protein. |
| 使用方法 | F(1:50) IF-IC (1:800) |
| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3). |
| 存放说明 | 4C |
Flow cytometric analysis of unpermeabilized HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate) (green) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).使用EpCAM (VU1D9) 鼠单抗（Alexa Fluor® 488标记）（绿色）对未渗透处理的HT-29细胞进行流失细胞分析，对照使用小鼠（MOPC-21）鼠单抗IgG1同型对照（Alexa Fluor® 488 标记）#4878（红色）。 | |
Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用EpCAM (VU1D9) 鼠单抗（Alexa Fluor® 488标记）（绿色）对HT-29细胞进行激光共聚焦荧光分析。肌动蛋白丝使用DY-554鬼笔环肽（红色）标记。蓝色假色=DRAQ5® #4084 （DNA荧光染料）。 |
