| 货号 | 12075S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human and rat cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.Cell Signaling Technology公司抗体偶联Alexa Fluor® 647荧光素染料并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联Alexa Fluor® 488的Ki-67 (D3B5)Rabbit mAb #9129显示同样的物种交叉反应。 |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | IF-F/IF-IC/F |
| 使用方法 | F(1:50) IF-F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).Ki-67，以其发现地命名（Kiel大学，德国），作为一个细胞核非组蛋白（1）通常在增殖的细胞中表达并且在休眠细胞中不表达（2）。Ki-67会让增殖细胞停滞在G1，S，G2和有丝分裂期，但是不会停滞在G0期。研究显示高表达的Ki-67与低乳腺癌存活率相关（3）。研究已经将Ki-67与其他的标记，作为乳腺癌和其他恶性疾病中的潜在的预后和预测标记（4）。 |
| 存放说明 | 4C |
Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #3465 (green). Actin filaments were labeled with DY-554 phalloidin (red).使用Ki-67 (D3B5)Rabbit mAb（偶联Alexa Fluor® 647）（蓝色假色）和Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb(偶联Alexa Fluor® 488) #3465 (绿色)对HeLa细胞进行激光共聚焦免疫荧光分析。 | |
Confocal immunofluorescent analysis of rat colon using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 Phalloidin #8878 (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. 使用Ki-67 (D3B5)Rabbit mAb（偶联Alexa Fluor® 647）（蓝色假色）对大鼠结肠组织进行激光共聚焦免疫荧光分析。肌动蛋白丝用Alexa Fluor® 488鬼笔环肽#8878（绿色）。 | |
Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 647 Conjugate) and DAPI #4083 (DNA content).使用Ki-67 (D3B5)Rabbit mAb（偶联Alexa Fluor® 647）和DAPI#4083(DNA content)对人Jurkat细胞进行流式分析。 |
