| 货号 | 8587S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated p21 Waf1/Cip1 (12D1) Rabbit mAb #2947.该抗体偶联于Alexa Fluor® 647 荧光染料，使用人类细胞进行直接流式细胞术和免疫荧光检测，通过内部测试。该抗体预计与未标记p21 Waf1/Cip1 (12D1) Rabbit mAb #2947有同样的种属交叉反应。 |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 使用方法 | F(1:50) IF-IC (1:800) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A is active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).肿瘤抑制蛋白p21 Waf1/Cip1能够充当细胞周期进程抑制剂。其功能与周期蛋白和周期蛋白依赖性激酶形成的异源三聚体存在化学计量关系。通过与CDK2复合体联合，它可以抑制激酶的活性和阻断G1/S进程(1)。然而，p21也可以增强CDK4或者CDEK6与周期蛋白-D形成的复合体的组装和活性(2)。p21的羧基末端区域能够充分结合并抑制PCNA，DNA聚合酶的一个亚基，而且可以协调细胞周期进程中DNA的复制(3)。基于紫外线损伤或者是在细胞周期中cdc2/cyclin B或CDK2/cyclin A活跃阶段，p53被磷酸化且通过p53-响应元件上调p21的表达(4)。泛素化和蛋白酶体降解能够下调p21的蛋白水平(5)。 |
| 存放说明 | 4C |
Flow cytometric analysis of Jurkat (blue) and MCF7 (green) cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate).流式细胞术检测Jurkat细胞（蓝色）和MCF7细胞（绿色），使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate)。 | |
Confocal immunofluorescent analysis of MCF7 cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #3465 (green). Actin filaments were labeled with DY-544 phalloidin (red).激光共聚焦免疫荧光方法检测MCF7细胞，使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate)，呈蓝色伪彩，及Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #3465，呈绿色。肌动蛋白纤维使用DY-544鬼笔环肽标记，呈红色。 |
