Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (Alexa Fluor® 488 Conjugate)
货号：
2834S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 2834S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb detects endogenous levels of Bcl-2 only when phosphorylated at Ser70. This antibody does not cross-react with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | Alexa Fluor 488 |
| 背景 | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of Jurkat cells, untreated (upper) or paclitaxel-treated (lower), using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (Alexa Fluor® 488 Conjugate) versus propidium iodide (DNA content). The blue inserts represent propidium iodide staining alone, showing cells in mitotic arrest in the paclitaxel-treated sample. 流式细胞仪分析未经处理(上图) 或经 paclitaxel处理(下图)的Jurkat细胞，所用抗体为Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (Alexa Fluor® 488 Conjugate）兔单抗，与 碘化（DNA 含量）做比对。蓝颜色的插入表示只有碘化染色, 图示为经过 paclitaxel 处理的有丝分裂终止的样本细胞。 |
