Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate)
货号：
12812S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 12812S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb #2197. Cell Signaling Technology公司抗体偶联藻红蛋白（PE）并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联的Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb #2197显示同样的物种交叉反应。 |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | F |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE? |
| 背景 | Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate). 使用Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate)对未处理（蓝色）或UV处理（绿色）的HeLa细胞进行流式分析。 |
