Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (PE Conjugate)
货号：
6485S 基本售价：
4060.0 元 规格：
100ul
产品信息
概述| 货号 | 6485S |
| 描述 | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710.CST公司生产的此抗体与藻红蛋白(PE)偶联，并通过人源细胞的流式细胞仪的测试。正如所CST期望，藻红蛋白偶联的抗体与未偶联的抗体Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710相同，都能够与相同的物种中的蛋白交叉反应。 |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | F |
| 目标/特异性 | Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (PE Conjugate) detects endogenous levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. It also detects Syk protein when singly phosphorylated at Tyr526 of human Syk or Tyr520 of mouse Syk. It does not cross-react with other tyrosine-phosphorylated protein tyrosine kinases. |
| 使用方法 | F(1:50) |
性能| 供应商 | CST |
| 标记 | PE |
| 背景 | Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7). Tyrosine 352 of Syk is involved in the association of PLC-γ1 (8). Tyrosines 525 and 526 are located in the activation loop of the Syk kinase domain, and phosphorylation of Tyr525/526 of human Syk (equivalent to the Tyr519/520 of mouse Syk) is essential for Syk function (9). |
| 存放说明 | 4C |
参考图片Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM and hydrogen peroxide (green), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (PE Conjugate).流式细胞仪分析Ramos细胞，未经过处理(蓝色)和经过anti-IgM 和过氧化氢处理(绿色)，所用抗体为Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (PE Conjugate)。 |
