| 货号 | 12081S |
| 描述 | This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710. |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | IF-IC/F |
| 目标/特异性 | Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. This antibody also detects Syk protein when singly phosphorylated at Tyr526 of human Syk or Tyr520 of mouse Syk. This antibody does not cross-react with other tyrosine-phosphorylated protein tyrosine kinases. |
| 使用方法 | F(1:50) IF-IC (1:50) |
| 供应商 | CST |
| 标记 | Alexa Fluor 647 |
| 背景 | Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9). |
| 存放说明 | 4C |
Flow cytometric analysis of Ramos cells, untreated (blue) or treated with anti-IgM and hydrogen peroxide (green), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor® 647 Conjugate).流式细胞术分析Ramos细胞，未处理(蓝色)或anti-IgM 和过氧化氢处理(绿色)，使用抗体是Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor® 647 Conjugate)。 | |
Confocal immunofluorescent analysis of Ramos cells untreated (left), or treated with anti-IgM (right), using Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.共聚焦分析Ramos细胞，未处理(左图)，或anti-IgM(右图)处理，使用抗体是Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Alexa Fluor® 647 Conjugate)(蓝色)。Red = Propidium Iodide (PI)/RNase Staining Solution #4087。 |
