| 货号 | 42749S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | CDK4 (D9G3E) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total CDK4 protein. In COS-7 cells, this antibody recognizes a 40 kDa background band of unknown origin. This antibody does not cross-react with CDK1, 2, or 6. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle (4). |
| 存放说明 | 4C |
| 参考文献 | 1 . Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81. 2 . Sherr, C.J. (1996) Science 274, 1672-7. 3 . Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25. 4 . Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9. |
Flow cytometric analysis of Jurkat cells using CDK4 (D9G3E) Rabbit mAb (PE Conjugate) and DRAQ5® #4084 (for DNA content). |
