| 货号 | 73382S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | F |
| 目标/特异性 | Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352. |
| 使用方法 | F(1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). |
| 存放说明 | 4C |
| 参考文献 | 1 . Chu, D.H. et al. (1998) Immunol Rev 165, 167-80. 2 . Iwashima, M. et al. (1994) Science 263, 1136-9. 3 . Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8. 4 . Chan, A.C. et al. (1995) EMBO J 14, 2499-508. 5 . Williams, B.L. et al. (1999) EMBO J 18, 1832-44. 6 . Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94. 7 . Wiestner, A. et al. (2003) Blood 101, 4944-51. 8 . Crespo, M. et al. (2003) N Engl J Med 348, 1764-75. |
Flow cytometric analysis of Ramos cells, untreated (blue) or IgM-treated (green), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (Alexa Fluor®488 Conjugate). |
