| 货号 | 9127S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from A431, COS and PC12 cells, untreated or nocodazole-treated, using Phospho-MEK1 (Thr286) Antibody (upper) or MEK1 Antibody #9124 (lower). | |
western blot方法检测细胞提取物：未处理和诺考达唑处理的A431, COS和PC12细胞，使用的抗体是Phospho-MEK1 (Thr286) Antibody 兔多抗(上图)和MEK1 Antibody #9124 兔多抗(下图)。 | |
Immunoprecipitation followed by Western blot analysis of extracts from COS cells, untreated or nocodazole-treated, using Phospho-MEK1 (Thr286) Antibody. | |
免疫沉淀实验之后用Western blot方法检测细胞提取物：未经处理的和诺考达唑处理的COS细胞，使用的抗体是Phospho-MEK1 (Thr286) Antibody兔多抗。 |
