| 货号 | 3519S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Phospho-Keratin 17 (Ser44) Antibody detects endogenous levels of keratin 17 only when phosphorylated on Ser44. |
| 使用方法 | WB(1:1000) F (1:50) |
| 供应商 | CST |
| 背景 | Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). |
| 存放说明 | -20C |
| 计算分子量 | 49 |
Western blot analysis of extracts of HeLa cells, either untreated or treated with nocodazole, using Phospho-Keratin 17 (Ser44) Antibody (upper) or Keratin 17 #3984 (lower).使用Phospho-Keratin 17 (Ser44) Antibody (上图)或Keratin 17 #3984 (下图)，免疫印迹（Western Blot）分析HeLa细胞中Phospho-Keratin 17和Keratin 17蛋白水平，细胞分为未处理组和nocodazole处理组。 | |
Flow cytometric analysis of HeLa cells using Phospho-Keratin17 (Ser44) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-Keratin17 (Ser77)-positive cells.与碘化丙碇（DNA含量）比较，使用Phospho-Keratin17 (Ser44) Antibody进行流式细胞仪分析HeLa细胞。圈在盒内的细胞群认为是phospho-Keratin17 (Ser77)阳性细胞。 |
