| 货号 | 2405S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | Phospho-HSP27 (Ser78) Antibody detects endogenous levels of HSP27 phosphorylated at Ser78 . This antibody does not cross-react with other phosphorylated heat shock proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:100) F (1:100) |
| 供应商 | CST |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
Western blot analysis of extracts from HeLa and COS cells, untreated, anisomycin-treated or UV-treated, using Phospho-HSP27 (Ser78) Antibody (upper) or HSP27 (G31) mAb #2402 (lower). 对HeLa和COS细胞抽提液，未处理或anisomycin处理或UV处理，使用Phospho-HSP27 (Ser78) Antibody（上图）或HSP27 (G31) mAb #2402（下图）进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-HSP27 (Ser78) Antibody. 对石蜡包埋的人乳腺癌使用Phospho-HSP27 (Ser78) Antibody进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-HSP27 (Ser78) Antibody. 对石蜡包埋的人肺癌使用Phospho-HSP27 (Ser78) Antibody进行免疫组化分析。 | |
Flow cytometric analysis of HeLa cells, untreated (blue) or UV treated (green), using Phospho-HSP27 (Ser78) Antibody compared to a nonspecific negative control antibody (red). 使用Phospho-HSP27 (Ser78) Antibody和非特异性阴性对照抗体（红色），未处理（蓝色）或用NGF处理（绿色），对HeLa细胞进行流式细胞仪分析。 |
