| 货号 | 4136S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) F (1:50) |
| 供应商 | CST |
| 背景 | Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).Cyclin E1和Cyclin E2协同并激活CDK2(1)。DNA损伤时，上调/激活CDK抑制基因p21 Waf1/Cip1 and p27 Kip1能够阻滞cyclin E/CDK2的激活，从而导致G1/S期停滞。当条件适宜于细胞周期连续进行时，cyclin D/CDK4/6使Rb磷酸化从而能够降低p21 Waf1/Cip1 和 p27 Kip1的活性，使得cyclin E/CDK2随后激活(1,2)。Cyclin E/CDK2进一步磷酸化Rb使细胞周期进入S期，在此期内cyclin E/CDK2被认为可以磷酸化并激活涉及DNA合成过程的多种蛋白(2,3)。周期蛋白E的功能转化主要受磷酸化控制，这种磷酸化能够导致SCFFbw7介导的泛素化和蛋白酶体依赖的降解(4,5)。Cyclin E1在体内可以在包括苏氨酸62位，丝氨酸88位，丝氨酸72位，苏氨酸380位和丝氨酸384位的多位点磷酸化，这个磷酸化过程至少可以被两个激酶-CDK2和GSK-3调控。 |
| 存放说明 | -20C |
| 计算分子量 | 48 |
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Cyclin E1 (Thr62) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-Cyclin E (Thr62)-positive cells. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using Phospho-Cyclin E (Thr62) Antibody.免疫组织化学方法检测石蜡包埋人类结肠癌组织，显示核定位，检测抗体为Phospho-Cyclin E (Thr62) Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-Cyclin E (Thr62) Antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Cyclin E (Thr62) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学方法检测石蜡包埋人类乳腺癌组织，左图为对照肽段，右图为抗原特异性肽段，检测抗体为Phospho-Cyclin E (Thr62) Antibody。 | |
Western blot analysis of extracts from 293 cells, treated with 10 mM LiCl for the indicated times, using Phospho-Cyclin E1 (Thr62) Antibody (upper) or a cyclin E antibody (lower). | |
Western blot analysis of extracts from 293 cells, untreated or hydroxyurea-treated, using Phospho-Cyclin E1 (Thr62) Antibody. |
