| 货号 | 14678S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5). |
| 存放说明 | -20C |
| 计算分子量 | 80 (NPM-ALK), 220 (ALK) |
Confocal immunofluorescent analysis of KARPAS-299 cells, untreated (left) or treated with Crizotinib #4401 (1 μM, 2hr; center), and HeLa cells (right), using Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge. | |
Flow cytometric analysis of KARPAS-299 cells, untreated (green) or treated with Crizotinib #4401 (1 μM, 2 hr, 37ºC; blue), using Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge. | |
Western blot analysis of extracts from KARPAS-299 cells, untreated (-) or treated with Crizotinib #4401 (1 μM, indicated times), using Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb (upper) and ALK (C26G7) Rabbit mAb #3333 (lower). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge. | |
Immunoprecipitation of phospho-ALK from KARPAS-299 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb. Western blot analysis was performed using Phospho-ALK (Tyr1507) (D6F1V) Rabbit mAb. KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge. |
