| 货号 | 13050S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 使用方法 | WB(1:1000) F (1:1600) |
| 供应商 | CST |
| 背景 | Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2A.X, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 350 |
Flow cytometric analysis of HeLa cells, untreated (left) or treated with camptothecin (1 μM, 2 hr; right), using Phospho-ATM (Ser1981) (D25E5) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. 未处理（左）或经过喜树碱（1 μM, 2 hr; 右）处理的HeLa细胞，使用Phospho-ATM (Ser1981) (D25E5) Rabbit mAb和碘化丙啶(PI)/RNase Staining Solution #4087对HeLa细胞进行流式分析以计算DNA量。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412用作二抗。 | |
Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated with neocarzinostatin (NCS 10 μM, 1 hr; +), using Phospho-ATM (Ser1981) (D25E5) Rabbit mAb (upper) and ATM (D2E2) Rabbit mAb #2873 (lower).未处理（-）或经过新抑癌蛋白（NCS 10 μM, 1 hr; +）处理的HCT 116细胞提取物，使用Phospho-ATM (Ser1981) (D25E5) Rabbit mAb (上)和 ATM (D2E2) Rabbit mAb #2873 (下)进行western blot分析。 |
