| 货号 | 4494S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb recognizes endogenous levels of DRP1 protein only when phosphorylated at Ser616. |
| 使用方法 | WB(1:1000) F (1:1600) IF-IC (1:3200) |
| 供应商 | CST |
| 背景 | Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease (reviewed in 6). |
| 存放说明 | -20C |
| 计算分子量 | 78-82 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or λ phosphatase-treated (right), using Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Phospho-DRP1 (Ser616) (D9A1)Rabbit mAb和碘化丙啶（DNA content）对未处理（左）或λ phosphatase-处理（右）HeLa细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated (+) with nocodazole (100 mg/ml, 17 hr), using Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb (upper) and DRP1 (D6C7) Rabbit mAb #8570 (lower).未处理（-）或经过nocodazole (100 mg/ml, 17 hr)处理（+）的HeLa细胞提取物，使用Phospho-DRP1 (Ser616) (D9A1)Rabbit mAb（上）和DRP1 (D6C7) Rabbit mAb #8570（下）进行western blot分析。 | |
Flow cytometric analysis of Jurkat cells, untreated (left) or λ phosphatase-treated (right), using Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb and propidium iodide (DNA content).使用Phospho-DRP1 (Ser616) (D9A1)Rabbit mAb和碘化丙啶（DNA content）对未处理（左）或λ phosphatase-处理（右）的Jurkat细胞进行流式分析。 |
