| 货号 | 12569S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb recognizes endogenous levels of Vimentin protein only when phosphorylated at Ser83. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell-specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentins dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7). |
| 存放说明 | -20C |
| 计算分子量 | 57 |
Confocal immunofluorescent analysis of HeLa cells using Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb对HeLa细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 (DNA content). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.使用Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb和碘化丙啶(PI)/RNase Staining Solution #4087 (DNA content)对未处理的Jurkat细胞进行流式分析。Anti-rabbit IgG (H+L), F(ab)2 fragment (Alexa Fluor® 488 conjugate) #4412作为二抗。 | |
Western blot analysis of extracts from HeLa cells, untreated (-), or treated with either hydroxyurea (4 mM, G1/S arrested; +), or Paclitaxel #9807 (100 nM, G2/M arrested; +) for 20 hr, using Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb (upper) and Vimentin (D21H3) XP® Rabbit mAb #5741 (lower).未处理(-),或经过hydroxyurea (4 mM, G1/S arrested; +),或Paclitaxel #9807 (100 nM, G2/M arrested; +)处理20小时的HeLa细胞提取物，使用Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb（上）和Vimentin (D21H3) XP® Rabbit mAb #5741（下）进行western blot分析。 |
