| 货号 | 13193S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 45-55 |
Flow cytometric analysis of human monocyte-derived dendritic cells stained with anti-human CD11c PE and either Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left) or DC-SIGN (D7F5C) XP® Rabbit mAb (right). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Immunoprecipitation of DC-SIGN from MUTZ-3 cell extracts differentiated into interstitial dendritic cells with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 7 d, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DC-SIGN (D7F5C) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DC-SIGN (D7F5C) XP® Rabbit mAb. | |
Western blot analysis of extracts from MUTZ-3 cells, undifferentiated (-) or differentiated (+) into interstitial dendritic cells with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 7 d, using DC-SIGN (D7F5C) XP® (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from human monocyte-derived dendritic cells using DC-SIGN (D7F5C) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of MUTZ-3 cells, undifferentiated (negative, left) or differentiated (positive, right) into interstitial dendritic cells with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 7 d, using DC-SIGN (D7F5C) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
