| 货号 | 5670S |
| 反应种属 | Human/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | HOP (D10E2) XP® Rabbit mAb recognizes endogenous levels of total HOP protein. This antibody is not known to cross-react with other heat shock proteins. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5). |
| 存放说明 | -20C |
| 计算分子量 | 64 |
Confocal immunofluorescent analysis of HeLa cells using HOP (D10E2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞使用HOP (D10E2) XP® Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Western blot analysis of whole cell extracts from various cell lines using HOP (D10E2) XP® Rabbit mAb. 对多种细胞系使用HOP (D10E2) XP® Rabbit mAb兔单抗进行Western blot分析。 | |
Flow cytometric analysis of untreated HeLa cells using HOP (D10E2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 使用HOP (D10E2) XP® Rabbit mAb兔单抗（蓝）和对应的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900，对未处理的HeLa细胞进行流式细胞术分析。 |
