| 货号 | 8455S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | IFN-γ (D3H2) XP® Rabbit mAb recognizes endogenous levels of total IFN-γ protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:400) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4). |
| 存放说明 | -20C |
| 计算分子量 | 17, 19, 23 |
Confocal immunofluorescent analysis of NK-92 cells, treated with TPA #4174 and Ionomycin #9995 (80 nM and 3 μM, 4 hr; left) or untreated (right), using IFN-γ (D3H2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对NK-92细胞进行共聚焦分析，TPA #4174 和 Ionomycin #9995 (80 nM and 3 μM, 4小时;左图) 或未处理 (右图)，使用抗体是IFN-γ (D3H2) XP® Rabbit mAb(绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from NK-92 cells, untreated (-) or treated (+) with TPA #4174 (80 nM, 5 hr), Ionomycin #9995 (3 μM, 5 hr), and Brefeldin A #9972 (300 ng/mL, last 4 hr of stimulation), using IFN-γ (D3H2) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析NK-92细胞的细胞提取物，未处理(-)或TPA #4174 (80 nM, 5 小时), Ionomycin #9995 (3 μM, 5小时), 和 Brefeldin A #9972 (300 ng/mL, 最后4小时刺激)处理(+)。使用抗体是IFN-γ (D3H2) XP® Rabbit mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Immunoprecipitation of IFN-γ from NK-92 cell extracts, treated with TPA #4174 (80 nM, 5 hr), Ionomycin #9995 (3 μM, 5 hr), and Brefeldin A #9972 (300 ng/mL, last 4 hr of stimulation), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IFN-γ (D3H2) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IFN-γ (D3H2) XP® Rabbit mAb.对NK-92细胞提取物中的IFN-γ进行免疫共沉淀，TPA #4174 (80 nM, 5 小时), Ionomycin #9995 (3 μM, 5小时), 和 Brefeldin A #9972 (300 ng/mL, 最后4小时刺激)处理，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) or IFN-γ (D3H2) XP® Rabbit mAb (泳道3).泳道1为10%的加入量。Western blot分析使用抗体是IFN-γ (D3H2) XP® Rabbit mAb。 |
