| 货号 | 12163S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | IL-10 (D13A11) Rabbit mAb recognizes endogenous levels of total IL-10 protein. |
| 使用方法 | WB(1:1000) F (1:400) |
| 供应商 | CST |
| 背景 | Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1), leading to the activation of Jak1 and Tyk2 and phosphorylation of Stat3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFN-γ receptor, by upregulating expression of SOCS1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Western blot analysis of extracts from purified CD14+ human peripheral blood mononuclear cells, untreated (-) or treated with LPS (100 ng/mL, 16 hr; +), using IL-10 (D13A11) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Brefeldin A #9972 (300 ng/mL) was added to cells after 1 hr of LPS stimulation.Western blot 分析纯化的CD4+人外周血单核细胞的细胞提取物，未处理(-)或LPS (100 ng/mL, 16小时; +)处理，使用抗体是IL-10 (D13A11) XP® Rabbit mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。Brefeldin A #9972 (300 ng/mL)在LPS刺激1h后被加入细胞中。 | |
Western blot analysis of extracts from purified CD4+ human peripheral blood mononuclear cells, activated with anti-CD3 and anti-CD28 in regulatory T cell inducing conditions for 12 d and then untreated (-) or treated (+) with TPA #4174 (40 nM, 6 hr), Ionomycin #9995 (3 μM, 6 hr), and Brefeldin A #9972 (300 ng/mL, last 5 hr of stimulation), using IL-10 (D13A11) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot 分析纯化的CD4+人外周血单核细胞的细胞提取物，使用调节性T细胞中的anti-CD3 和anti-CD28激活诱导12天，接着未处理（-）或TPA #4174 (40 nM, 6 小时)，Ionomycin #9995 (3 μM, 6小时), 和 Brefeldin A #9972 (300 ng/mL, 最后5小时刺激)处理(+)，使用抗体是IL-10 (D13A11) XP® Rabbit mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Western blot analysis of recombinant Human Interleukin-10 (hIL-10) #8903 using IL-10 (D13A11) XP® Rabbit mAb.Western blot分析recombinant Human Interleukin-10 (hIL-10) #8903，使用抗体是IL-10 (D13A11) XP® Rabbit mAb。 | |
Flow cytometric analysis of purified CD14+ human peripheral blood mononuclear cells untreated (left) or treated with LPS (100ng/mL, 16 hr; right) using a CD14 antibody and IL-10 (D13A11) XP® Rabbit mAb. Brefeldin A #9972 (300ng/mL) was added to untreated and treated cells after 1 hr of LPS stimulation. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.流式细胞术分析纯化的CD4+人外周血单核细胞的细胞提取物，未处理(左图)或LPS (100 ng/mL, 16小时; 右图)处理，使用抗体是一种CD14抗体和IL-10 (D13A11) XP® Rabbit mAb。Brefeldin A #9972 (300 ng/mL)在LPS刺激1h后被加入细胞中。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414用来作为二抗。 | |
Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or treated (right) with TPA #4174 (40 nM, 5 hr), Ionomycin #9995 (2 μM, 5 hr) and Brefeldin A #9972 (1 μg/mL, last 4 hr of stimulation), using a CD3 antibody and IL-10 (D13A11) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. Analysis was performed on cells in the lymphocyte gate.流式细胞术分析人外周血单核细胞，未处理（左图）或TPA #4174 (40 nM, 5小时), Ionomycin #9995 (2 μM, 5 小时) 和 Brefeldin A #9972 (1 μg/mL, 最后刺激4h)处理（右图），使用抗体是一种CD3抗体和IL-10 (D13A11) XP® Rabbit mAb. Anti-rabbit IgG (H+L)，F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414用来作为二抗。分析基于淋巴细胞门上细胞进行。 |
