| 货号 | 4465S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Keratin 7 (D1E4) XP® Rabbit mAb recognizes endogenous levels of total keratin 7 protein. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
Western blot analysis of extracts from HeLa and MCF7 cells using Keratin 7 (D1E4) XP® Rabbit mAb. Note that MCF7 cells are negative for Keratin 7, as expected.使用Keratin 7 (D1E4) XP®Rabbit mAb对HeLa细胞和MCF7细胞提取物进行western blot分析。注意如预期的，MCF7是Keratin7阴性的。 | |
Confocal immunofluorescent analysis of HeLa (left) and MCF7 (right) cells using Keratin 7 (D1E4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Keratin 7 (D1E4) XP® Rabbit mAb（绿色）对HeLa（左）和MCF7（右）细胞进行激光共聚焦免疫荧光分析。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of MCF7 (blue) and HeLa (green) cells using Keratin 7 (D1E4) XP® Rabbit mAb.使用Keratin 7 (D1E4) XP®Rabbit mAb对MCF7（蓝色）和HeLa（绿色）进行流式分析。 |
