| 货号 | 3695S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
Western blot analysis of extracts of NTERA-2 and IGROV1 cells using LIN28A (D84C11) XP® Rabbit mAb. 使用LIN28A (D84C11) XP® 兔单抗对NTERA-2和IGROV1细胞提取物进行western blot分析。 | |
Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using LIN28A (D84C11) XP® Rabbit mAb. 使用LIN28A (D84C11) XP®兔单抗对HeLa细胞（蓝色）和NTERA2细胞（绿色）进行流式细胞分析。 | |
Confocal immunofluorescent analysis of NTERA-2 cells using Lin28A (D84C11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用LIN28A (D84C11) XP® 兔单抗（绿色）对NTERA-2细胞进行激光共聚焦荧光分析。肌动蛋白丝使用Alexa Fluor® 554 phalloidin（红色）标记。蓝色伪彩=DRAQ® #4084 (DNA荧光染料). |
