货号 | 3686S |
反应种属 | Human/Rat |
来源宿主 | Rabbit |
应用 | W/IP/IF-IC/F |
使用方法 | WB(1:1000) IP (1:200) F (1:800) IF-IC (1:1600) |
供应商 | CST |
背景 | p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin 2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).p27 Kip1是周期蛋白依赖性激酶抑制剂Cip/Kip家族的一员。同其类似物一样,如p57 Kip2 和p21 Waf1/Cip1,其巩固G1限制点的能力源于CDK2/cyclin E和其它CDK/cyclin复合体的结合抑制。在静止期细胞和cAMP或其它细胞周期阴性调节剂处理的细胞中,p27的表达是上调的。白细胞介素2或者其它有丝分裂原能够诱导p27的下调;该过程涉及p27的磷酸化和泛素化-蛋白酶体途径介导的降解(1-4)。 |
存放说明 | -20C |
计算分子量 | 27 |
Western blot analysis of extracts from various cell types using p27 Kip1 (D69C12) XP® Rabbit mAb.Western blot 检测不同细胞提取物,使用的抗体为p27 Kip1 (D69C12) XP® Rabbit mAb。 | |
Immunoprecipitation of p27 Kip1 from 293 cells using p27 Kip1 (D69C12) XP® Rabbit mAb. Western analysis was performed using the same antibody. Lane 1 is 5% input.从293细胞中免疫沉淀p27 Kip1 蛋白,使用的抗体为p27 Kip1 (D69C12) XP® Rabbit mAb。使用同一抗体进行Western blot检测。 | |
Confocal immunofluorescent analysis of MCF-7 cells using p27 Kip1 (D69C12) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测MCF-7细胞,使用的抗体为p27 Kip1 (D69C12) XP® Rabbit mAb,呈绿色。肌动蛋白纤维由DY-554鬼笔环肽标记,呈红色。 | |
Flow cytometric analysis of Jurkat cells using p27 Kip1 (D69C12) XP® Rabbit mAb versus Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |