| 货号 | 36746S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | IP IF-IC F |
| 目标/特异性 | EpCAM (D4K8R) XP® Rabbit mAb recognizes endogenous levels of total EpCAM protein. |
| 使用方法 | IP(1:50) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Went, P.T. et al. (2004) Hum Pathol 35, 122-8. 2 . Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23. 3 . Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6. |
Flow cytometric analysis of fixed and unpermeabilized (left) and live (right) HeLa cells (blue), HT-29 cells (red), and MCF7 cells (green) using EpCAM (D4K8R) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of HT-29 (left) and HeLa (right) cells using EpCAM (D4K8R) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note that EpCAM (D4K8R) XP® Rabbit mAb has been shown to detect its target protein on live, fixed, and fixed/permeabilized cells. |
