| 货号 | 5365S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | PSA/KLK3 (D6B1) XP® Rabbit mAb recognizes endogenous levels of total PSA/KLK3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 N-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 29 |
Western blot analysis of extracts from LNCaP (PSA positive) and DU 145 (PSA negative) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot检测LNCaP (PSA 阳性)和DU 145 (PSA 阴性)细胞的提取物，使用的抗体是PSA/KLK3 (D6B1) XP® Rabbit mAb (上图) 或者β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Flow cytometry analysis of DU 145 (negative, blue) and LNCaP (positive, green) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb. 流式细胞术分析DU 145 （阴性，蓝色)和LNCaP (阳性，绿色)细胞，使用的抗体是PSA/KLK3 (D6B1) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of LNCaP (positive, left) or DU 145 (negative, right) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).j激光共聚焦免疫荧光分析LNCaP (阳性，左图)或DU 145 (阴性，右图)细胞，使用的抗体是PSA/KLK3 (D6B1) XP® Rabbit mAb (绿色)，蓝色伪彩 = DRAQ5® #4084 (荧光DNA染料)。 |
