| 货号 | 4936S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 目标/特异性 | Wee1 Antibody detects endogenous levels of Wee1 protein independent of phosphorylation. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-8).所有真核细胞进入有丝分裂都经由cdc2激酶激活的调节。激活cdc2进入有丝分裂进程的关键的调节步骤似乎是15位和14位酪氨酸的去磷酸化（1,2）。15位和14位酪氨酸的磷酸化和cdc2抑制是通过Wee1和MYT1蛋白激酶来执行的，而15位酪氨酸的去磷酸化和cdc2活化是通过cdc25磷酸酶来执行（1,3,4）。有丝分裂中Myt1的过度磷酸化表明G2/ M转换期一个或多个激酶的激活负向调节Myt1活性。已经证实的磷酸化Myt1的激酶包括cdc2，p90RSK，Akt和Plk1 (5-8)。 |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Watanabe, N. et al. (1995) EMBO J 14, 1878-91. 2 . Hunter, T. (1995) Cell 80, 225-36. 3 . Galaktionov, K. et al. (1995) Genes Dev 9, 1046-58. 4 . McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85. 5 . Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6. 6 . Palmer, A. et al. (1998) EMBO J 17, 5037-47. 7 . Palmer, A. et al. (1998) EMBO J 17, 5037-47. 8 . Nakajima, H. et al. (2003) J Biol Chem 278, 25277-80. 9 . Parker, L.L. et al. (1995) Proc Natl Acad Sci U S A 92, 9638-42. 10 . Watanabe, N. et al. (2004) Proc Natl Acad Sci U S A 101, 4419-24. |
Western blot analysis of extracts from HeLa cells, untreated or Lambda Phosphatase-treated NEB #P0753 (10,000 units/ml for 1h), using Wee1 Antibody.Western blot方法检测未处理和lamda磷酸酶处理 (10,000 units/ml，NEB #P0753 )1小时的Hela细胞，使用的抗体为Wee1 Antibody。 | |
Western blot analysis, using Wee1 Antibody, of extracts from HeLa and 293 cells (lanes 1 and 3) and of protein immunoprecipitated with Wee1 Antibody from the same lysates (lanes 2 and 4).Western blot方法检测Hela细胞和293细胞提取物（泳道1和3）及Wee1抗体从同种裂解液中免疫沉淀的蛋白（泳道2和4），使用的抗体为Wee1 Antibody。 | |
Flow cytometric analysis of untreated Jurkat cells, using Wee1 Antibody versus propidium iodide (DNA content). The boxed population indicates Wee1-positive cells.流式细胞术检测未处理的Jurkat细胞，使用的抗体为Wee1 Antibody，碘化乙啶检测DNA含量作为对照。框内的细胞群为Wee1阳性细胞。 |
