SHIP1 (P290) Antibody【科瑞奥博】

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SHIP1 (P290) Antibody

货号： 2726S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	2726S
反应种属	Human
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	SHIP1 (P290) Antibody detects endogenous levels of total SHIP1 protein.
使用方法	WB(1:1000) IP (1:100) F (1:50) IF-IC (1:25)

性能
供应商	CST
灵敏度	Endogenous
背景	SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6).
存放说明	-20C
计算分子量	145
参考文献	1 . Tridandapani, S. et al. (1997) Mol. Cell. Biol. 17, 4305-4311. 2 . Liu, L. et al. (1997) J Biol Chem 272, 8983-8. 3 . Malbec, O. et al. (2001) J Biol Chem 276, 30381-91. 4 . Carver, D.J. et al. (2000) Blood 96, 1449-56. 5 . Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72. 6 . Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.

参考图片

Flow cytometric analysis of RL cells, using SHIP1 (P290) Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞仪分析 RL 细胞，所用抗体为SHIP1 (P290) Antibody (蓝色)，与非特异性的阴性对照抗体对比(红色)。

Western blot analysis of total cell lysate from RL cells, using SHIP1 (P290) Antibody.Western免疫印迹。用SHIP1 (P290) Antibody研究RL细胞总的细胞提取液。

Confocal immunofluorescent analysis of THP-1 (left) and Jurkat cells (right), using SHIP1 (P290) Antibody (red) and CD3 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析THP-1细胞 (左图) 和Jurkat细胞 (右图) ，所用抗体为SHIP1 (P290) Antibody(红色)和CD3 Antibody(绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。