RPA70/RPA1 Antibody【科瑞奥博】

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RPA70/RPA1 Antibody

货号： 2267S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	2267S
反应种属	Human/Monkey
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	RPA70 antibody detects endogenous levels of total RPA70 subunit.
使用方法	WB(1:1000) IP (1:50) F (1:25) IF-IC (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol α, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12).RPA70 (HSSB, REPA1, RF-A, RP-A, p70)是异源三聚体复合物的组成部分，由70、32/30和14 kDa的亚基组成，统称RPA。RPA是一种单链DNA结合蛋白，其DNA结合活性完全位于70 kDa的亚基。几乎是所有的DNA代谢过程如DNA复制(1-3)、重组、细胞周期和DNA损伤检验点以及所有主要类型的DNA修复包括核苷酸切除、碱基切除、错配和双链断裂修复都需要该复合体的参与(4-7)。应答真核细胞基因毒性应激反应的过程中，RPA被认为可以协同Rad9/Rad1/Hus1 (9-1-1)检验点复合体(8)。DNA损伤时或者检验点激酶包括ATM、ATM-Rad3、ATR和DNA-依赖性蛋白激酶(DNA-PK) (9-11)引起的复制应激中，RPA是过度磷酸化的。过度磷酸化可以改变RPA-DNA和RPA-蛋白相互作用。除检验点伴侣之外，RPA能够和多种蛋白伴侣相互作用，包括正常复制所需蛋白如RCF，PCNA和Pol α以及参与SV40复制的蛋白如DNA聚合酶I和SV40大T抗原(10,12)。
存放说明	-20C
计算分子量	70
参考文献	1 . Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222-7231. 2 . Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834-1838. 3 . Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211-1218. 4 . Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523-2527. 5 . Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433-9. 6 . Kastan, M.B. and Bartek, J. (2004) Nature 432, 316-323. 7 . Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39-85. 8 . Guo, S. et al. (2006) J Biol Chem 281, 21607-16. 9 . Wu, X. et al. (2005) Oncogene 24, 4728-4735. 10 . Binz, S.K. et al. DNA Repair (Amst) 3, 1015-1024. 11 . Nuss, J.E. et al. (2005) Biochemistry 44, 8428-8437. 12 . Yuzhakov, A. et al. (1999) EMBO J. 18, 6189-6199.

参考图片

Western blot analysis of extracts from A431 and K562 cells, using RPA70 Antibody.Western blot 方法检测A431和K562细胞提取物，使用的抗体为RPA70 Antibody。

Confocal immunofluorescent images of HeLa cells, untreated (left) or UV-treated (right), labeled with RPA70 Antibody (green) showing translocation to distinct nuclear foci after UV damage. Actin filaments have been labeled with Alexa Fluor® 555 phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).激光共聚焦荧光法检测HeLa 细胞，左图为未处理的，右图为紫外处理的，使用RPA70 Antibody (绿色)展示紫外诱导损伤后不同核灶的迁移情况。肌动蛋白纤维使用 Alexa Fluor® 555 鬼笔环肽标记。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。

Flow cytometric analysis of Jurkat cells, using RPA70 antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞术检测Jurkat 细胞，使用的抗体为(蓝色)，相比较于非特异性阴性对照(红色)。