| 货号 | 3729S |
| 反应种属 | Human/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | OSR1 Antibody detects endogenous levels of total OSR1 protein. This antibody does not cross-react with SPAK or other members of the GCK family. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:25) |
| 供应商 | CST |
| 背景 | SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitro studies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3, 4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Flow cytometric analysis of HeLa cells using OSR1 Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (red)比较，使用OSR1 Antibody (蓝色)标记，流式细胞仪分析HeLa细胞。 | |
Confocal immunofluorescent analysis of HeLa cells using OSR1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用OSR1 Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(red)。蓝色伪彩= DRAQ5® #408（DNA荧光染料）。 | |
Western blot analysis of extracts from 293, HeLa, Jurkat and COS cells using OSR1 Antibody. 使用OSR1 Antibody，免疫印迹（Western Blot）分析293、HeLa、Jurkat和COS细胞中OSR1蛋白水平。 |
