| 货号 | 2753S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:25) IHC-P (1:300) F (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks (2-5). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). DNA-PK has been shown to phosphorylate many proteins, including p53, serum response factor, c-Jun, c-Fos, c-Myc, Oct-1, Sp-1 and RNA polymerase II (1,8). The combined activities of Ku70/Ku80 and DNA-PK implicate Ku in many cellular functions, including cell cycle regulation, DNA replication and repair, telomere maintenance, recombination and transcriptional activation.Ku是由两个亚基(Ku70 and Ku80)组成的异源二聚体蛋白，最初在几种自体免疫疾病包括硬皮病、多发性肌炎和系统性红斑狼疮中作为自体抗原被鉴定出来(1)。Ku是一种高丰度、广泛表达的核蛋白，它能够结合并稳定端粒或双链DNA断裂处的DNA末端(2-5)。Ku70/Ku80异源二聚体具有ATP-依赖的DNA解旋酶活性，并能够作为DNA-依赖蛋白激酶(DNA-PK)的DNA-结合调节成分发挥作用(6-8)。DNA末端DNA-PK复合体的装配需要非同源末端连接(NHEJ)，一种涉及双链DNA断裂修复和V(D)J重组的机制(8)。DNA-PK能够磷酸化许多蛋白，包括p53，血清反应因子，c-Jun，c-Fos，c-Myc，Oct-1，Sp-1 和RNA 聚合酶 II (1,8)。Ku70/Ku80和DNA-PK的联合活性暗示了Ku在多种细胞功能中的作用，包括细胞周期调控、DNA复制和修复、端粒维持、重组和转录激活。 |
| 存放说明 | -20C |
| 计算分子量 | 86 |
Western blot analysis of lysates from HeLa, A673, A549 and COS cells, using Ku80 antibody.Western blot方法检测HeLa, A673, A549 和 COS细胞提取物，使用的抗体为 Ku80 antibody。 | |
DAPI staining (left) and immunofluorescent staining (right) of paraformaldehyde-fixed HeLa cells, using Ku80 Antibody.对甲醛固定的HeLa细胞分别进行DAPI染色（左图）和免疫荧光染色（右图），使用的抗体为Ku80 Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Ku80 Antibody preincubated with control peptide (left) or antigen-specific peptide (right).免疫组织化学方法检测石蜡包埋人类乳腺癌组织，显示核定位，预孵育对照多肽（左图）或抗原特异性多肽（右图），使用的抗体为Ku80 Antibody 。 | |
Immunohistsochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localiztion, using Ku80 Antibody.免疫组织化学方法检测石蜡包埋人类肺癌组织，显示核定位，使用的抗体为Ku80 Antibody 。 | |
Flow cytometric analysis of HeLa cells, using Ku80 Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞术分析HeLa细胞，使用的抗体为Ku80 Antibody ，呈蓝色，非特异性阴性对照抗体作为对照，为红色。 |
