| 货号 | 5122S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | FAM129B/Niban-like protein 1 (family with sequence similarity 129, member B) belongs to a poorly characterized family of Niban proteins that also includes FAM129A/Niban and FAM129C/Niban-like protein 2. FAM129A/Niban is implicated in the ER stress response and is upregulated at the protein level in thyroid carcinoma (1,2). FAM129C/Niban-like protein 2 is preferentially expressed in B-cells and is one of five biomarkers upregulated in whole blood from patients with colorectal carcinoma (3,4). FAM129B is broadly expressed and has been shown to be a downstream target of B-Raf in melanoma cells (5). Though FAM129B does not appear to regulate cell growth and division, phosphorylation of FAM129B by B-Raf is essential for the invasive potential of melanoma and non-melanoma cell lines (5). Deletion of FAM129B in melanoma cells significantly impairs B-Raf/MEK/Erk-dependent invasion into the extracellular matrix (5). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Western blot analysis of whole cell extracts from various cell lines using FAM129B Antibody (upper) or GAPDH (14C10) Rabbit mAb Antibody #2118 (lower).Western blot方法检测所有细胞提取物：各种细胞系，使用的抗体是FAM129B Antibody (上图)和GAPDH (14C10) Rabbit mAb Antibody #2118 (下图)。 | |
Confocal immunofluorescent analysis of A-431 (left) and HeLa cells (right) using FAM129B Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦荧光法检测：A-431 细胞（左侧）和HeLa细胞（右侧）。使用的抗体是FAM129B Antibody (green)。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 | |
Flow cytometric analysis of HeLa cells using FAM129B Antibody (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).Flow cytometric方法检测细胞提取物：HeLa细胞，使用的抗体是FAM129B Antibody (蓝色) ，红色表示使用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900。 |
