| 货号 | 4727S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | c-Rel Antibody detects endogenous levels of total c-Rel protein. Cross reactivity was not detected with other family members. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:400) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from K562, Raji and 293 cells, using c-Rel Antibody.Western免疫印迹。用c-Rel Antibody研究K562, Raji 和 293 细胞的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using c-Rel Antibody in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学染色分析石蜡包埋人乳腺癌组织。在对照多肽(左图) 或 抗原特异性封闭多肽(右图)的存在下所用抗体为c-Rel Antibody 。 | |
Immunohistochemical analysis of paraffin-embedded Hodgkins lymphoma, showing nuclear and cytoplasmic localization using c-Rel Antibody.免疫组织化学染色分析石蜡包埋人霍奇金淋巴瘤组织，图示为细胞核和细胞质的定位。所用抗体为c-Rel Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human skin, using c-Rel Antibody.免疫组织化学染色分析石蜡包埋人皮肤组织。所用抗体为c-Rel Antibody。 | |
Flow cytometric analysis of K562 cells using c-Rel Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究K562细胞，所用抗体为c-Rel Antibody (蓝色) 与非特异性的阴性对照抗体(红色)。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TNFα-treated (#2169, 20 ng/ml for 20 min, right), using c-Rel Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).共聚焦免疫荧光分析未经处理(左图)和经TNFα(#2169, 20 ng/ml,20 min, 右图)处理的HeLa细胞，所用的抗体为c-Rel Antibody (绿色)。 肌丝蛋白用DY-554 phalloidin (红色)标记。 |
