| 货号 | 9502S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 目标/特异性 | Caspase-9 Antibody detects endogenous levels of full length caspase-9 (47 kDa) and large fragments of caspase-9. The antibody does not recognize other caspases. |
| 使用方法 | WB(1:1000) F (1:100) |
| 供应商 | CST |
| 背景 | Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). |
| 存放说明 | -20C |
| 计算分子量 | 35, 37, 47 |
Western blot analysis of HeLa cells, untreated, staurosporine-treated (1 µM) or cytochrome c-treated (0.25 mg/ml), and Jurkat cells, untreated or cytochrome c-treated, using Caspase-9 Antibody (Human Specific). Western blot分析未处理、staurosporine(星胞菌素)(1 µM) 或cytochrome(细胞色素C)(0.25 mg/ml)处理的HeLa 细胞，及未处理、或细胞色素C 处理的Jurkat细胞 ，抗体采用Caspase-9 Antibody (人特异性)。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Caspase-9 Antibody (Human Specific) compared to a nonspecific negative control antibody (red). 流式细胞术分析:未处理（蓝色） 或 etoposide (依托泊苷)处理的Jurkat 细胞，抗体采用Caspase-9 Antibody (人特异性)，并用非特异性抗体做阴性对照（红色）。 |
