| 货号 | 4334S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | AML1 antibody detects endogenous levels of total AML1 protein and the AML1/ETO fusion protein. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Flow cytometric analysis of Jurkat cells, using AML1 Antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞仪分析Jurkat细胞，所用抗体为AML1 Antibody(蓝色)与非特异性的对照抗体对比 (红色)。 | |
Western blot analysis of extracts from Jurkat and Kasumi-1 cells, using AML1 Antibody.Western免疫印迹。用AML1 Antibody研究Jurkat和Kasumi-1 细胞的细胞提取液。 | |
Confocal immunofluorescent image of Jurkat cells labeled with AML1 Antibody (green). Mitochondria have been labeled with MitoTracker Red CMXRos.共聚焦免疫荧光分析Jurkat 细胞，所用抗体为AML1 Antibody (绿色)。 线粒体用MitoTracker Red CMXRos标记。 |
