| 货号 | 13662S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:50) |
| 供应商 | CST |
| 背景 | VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3). |
| 存放说明 | -20C |
| 计算分子量 | 95-120 |
Flow cytometric analysis of fixed, non-permeabilized HUVEC cells, untreated (blue) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr; green) using VCAM1 (E1E8X) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor®488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human VCAM-1 (hVCAM-1-Myc/DDK; +), using VCAM-1 (E1E8X) Rabbit mAb. | |
Western blot analysis of extracts from HUVEC cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (10 ng/ml, 16 hr; +), using VCAM-1 (E1E8X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
