| 货号 | 13180S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | 4F2hc/CD98 (D6O3P) Rabbit mAb recognizes endogenous levels of total 4F2hc/CD98 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:400) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | 4F2hc is a transmembrane protein that belongs to the solute carrier family. 4F2hc forms heterodimeric complexes with various amino acid transporters such as LAT1 and LAT2 and regulates uptake of amino acids (1-5). 4F2hc is one of the earliest expressed antigens on the surface of activated human lymphocytes (6), hence it is also named CD98. 4F2hc is expressed in all cell types with the exception of platelets, and is expressed at highest levels in the tubules of the kidney and the gastrointestinal tract (7,8). It is localized at the plasma membrane when associated with LAT1 or LAT2 (9) and at the apical membrane of placenta (10). Research studies have shown that 4F2hc is highly expressed in various tumors including glioma (11), ovarian cancer (12), and astrocytomas (13), and it has been implicated in tumor progression and correlated with poor outcome in patients with pulmonary neuroendocrine tumors (14). 4F2hc is also involved in integrin trafficking through association with β1 and β4 integrins, and regulates keratinocyte adhesion and differentiation (15). |
| 存放说明 | -20C |
| 计算分子量 | 75-120 |
Confocal immunofluorescent analysis of HeLa (left) and SW620 (right) cells using 4F2hc/CD98 (D6O3P) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用4F2hc/CD98 (D6O3P) Rabbit mAb兔单抗 (绿色)对HeLa(左)和SW620(右)进行激光共聚焦免疫荧光分析。蓝色伪彩= DRAQ5® #4084 (fluorescent DNA dye)。 | |
Flow cytometric analysis of HeLa cells using 4F2hc/CD98 (D6O3P) Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 conjugate) #4412 was used as a secondary antibody. 使用4F2hc/CD98 (D6O3P) Rabbit mAb 兔单抗(蓝色) 对照Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)对HeLa细胞进行流式分析。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor®488 conjugate) #4412用作二抗。 | |
Western blot analysis of extracts from ZR-75-1 and HeLa cells using 4F2hc/CD98 (D6O3P) Rabbit mAb. 使用4F2hc/CD98 (D6O3P) Rabbit mAb兔单抗对ZR-75-1和HeLa细胞提取物进行western blot分析。 | |
Immunoprecipitation of 4F2hc/CD98 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or 4F2hc/CD98 (D6O3P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using 4F2hc/CD98 (D6O3P) Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或4F2hc/CD98 (D6O3P) Rabbit mAb 兔单抗(lane 3)对HeLa细胞提取物中的4F2hc/CD98进行免疫沉淀实验。Lane1是10%上样量。使用4F2hc/CD98 (D6O3P) Rabbit mAb兔单抗进行western blot分析。 |
