| 货号 | 14475S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
Confocal immunofluorescent analysis of HeLa cells using Aurora A (D3E4Q) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of Jurkat cells using Aurora A (D3E4Q) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from HT-29 cells, untreated (-) or synchronized in mitosis by treatment with thymidine (2 mM, 17 hr; +) and Nocodazole #2190 (100 ng/ml, 24 hr; +), using Aurora A (D3E4Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of Aurora A from HeLa cell extracts using Rabbit (Da1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Aurora A (D3E4Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Aurora A (D3E4Q) Rabbit mAb. |
