| 货号 | 12109S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) |
| 供应商 | CST |
| 背景 | The eukaryotic cell cycle is carefully controlled by protein phosphorylation involving a number of phosphatases, kinases, and co-factors. Cyclin-dependent kinases (CDKs/cdcs), Polo-like kinases (PLKs), and Aurora kinases have been shown to be major regulators of mitotic control (reviewed in 1,2). Protein aurora borealis (Bora), a co-factor of Aurora-A first identified in Drosophila, also plays a key roll in cell cycle progression (3). Bora levels are low in G0/G1, increasing in S-phase and peaking at G2 (4). Found to be conserved from C. elegans to humans, Bora is translocated from the nucleus to the cytoplasm upon activation of cdc2 at the onset of mitosis. Once present in the cytoplasm, Bora binds to and activates Aurora-A and PLK1 (3-5). It has been proposed that the binding of human Bora to PLK1 may lead to a conformational change in the protein that disrupts the autoinhibition by the Polo-Box Domain (PBD). This would allow for Thr210 on PLK1 to become more accessible for phosphorylation by Aurora-A (reviewed in 6). Active PLK1 then initiates the PLK1-cdc25-cdc2 positive feedback loop, leading to mitotic entry and the phosphorylation of Bora. Once phosphorylated in prophase, Bora is degraded allowing for normal mitotic progression (7).涉及一系列磷酸酯酶，激酶和共同因子的蛋白磷酸化精密的调控了真核细胞周期。研究表明细胞周期依赖性激酶(CDKs/cdcs), Polo-like激酶（PLKs）和Aurora激酶是有丝分裂调控的关键因子（1,2）。Protein aurora borealis (Bora),最初在果蝇内发现的一种Aurora-A的协同因子，也在细胞周期过程中发挥了重要作用（3）。Bora的表达量在G0/G1期较低，在S-期开始增加并在G2期达到峰值（4）。Bora蛋白在线虫到人类都是保守的，有丝分裂起始时cdc2的激活作用能够使Bora蛋白从细胞核转移到细胞质。一旦出现在细胞质后，Bora会结合并激活Aurora-A和PLK1(3-5).有研究提出假设人Bora蛋白与PLK1结合可能会导致蛋白构象改变破坏Polo-Box结构域（PBD）的自抑制。这将使PLK1的210位苏氨酸更里被Aurora-A磷酸化（6）。激活的PLK1随后起始了PLK1-cdc25-cdc2的正反馈，引发细胞进入有丝分裂以及Bora的磷酸化。Bora一旦在前期被磷酸化后，将被正常的有丝分裂进程所降解（7）。 |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from various cell lines using Bora (D2B9) Rabbit mAb.使用Bora (D2B9)Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of Bora from Jurkat cell extracts using Bora (D2B9) Rabbit mAb. Western blot detection was performed using the same antibody. Lane 1 is 10% input.使用Bora (D2B9)Rabbit mAb对Jurkat细胞的Bora蛋白进行免疫沉淀实验。Western blot检测使用同样的抗体。Lane1是10%上样量。 | |
Flow cytometric analysis of Jurkat cells using Bora (D2B9) Rabbit mAb compared to DNA content (Propidium Iodide (PI)/RNase Staining Solution #4087), showing peak bora expression in G2 phase. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.使用Bora (D2B9)Rabbit mAb对比DNA content（碘化丙啶(PI)/RNase 固定液 #4087）对Jurkat细胞进行流式细胞分析，以展示G2期的bora表达峰值情况。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412作为二抗使用。 |
