| 货号 | 5640S |
| 反应种属 | Human/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | CD44 (8E2) Mouse mAb detects endogenous levels of total CD44 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:800) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from HeLa, C6 and HUVEC cells using CD44 (8E2) Mouse mAb.Western免疫印迹。用CD44 (8E2) Mouse mAb研究HeLa, C6 和 HUVEC 细胞的细胞提取液。 | |
Projected confocal z-stack of HeLa cells using CD44 (8E2) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).投射共聚焦分析z-stack 的HeLa细胞，所用抗体为CD44 (8E2) Mouse mAb (绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Flow cytometric analysis of Hela cells using CD44 (8E2) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞仪分析Hela细胞，所用抗体为CD44 (8E2) Mouse mAb (蓝色) 与非特异性对照抗体对比(红色)。 |
