| 货号 | 58982S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | CENP-F (mitosin), is a kinetochore-associated protein whose expression and localization to chromatin is regulated in a cell cycle-dependent manner, with its highest expression in G2/M phases (1, 2). CENP-F is required for appropriate localization of other kinetocore-associated proteins, including CENP-E. CENP-F regulates kinetocore function and maintenance of the mitotic spindle checkpoint. Farnesylation of CENP-F is required for its localization and function (3). CENP-F also interacts with the mitochondrial protein, miro, to direct the distribution of mitochondria to daughter cells as they exit mitosis (4). Researchers have shown that CENP-F drives prostate tumor growth synergistically with FOXM1 in human and mouse (5), and that the gene for CENP-F is among those frequently amplified in hepatocellular, head and neck, and esophageal carcinomas (6-8). CENP-F expression has also been shown in research studies to be associated with poor prognosis in breast cancer (9). |
| 存放说明 | -20C |
| 计算分子量 | 350 |
Flow cytometric analysis of Jurkat cells using CENP-F (D6X4L) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of HT-29 cells, untreated (-) or synchronized in mitosis by treating with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using CENP-F (D6X4L) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Confocal immunofluorescent analysis of HT-29 cells using CENP-F (D6X4L) Rabbit mAb (green) and a Cyclin B1 antibody (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of HT-29 cells at various stages of mitosis using CENP-F (D6X4L) Rabbit mAb (green). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye). |
