| 货号 | 3308S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | c-Kit (Ab81) Mouse mAb detects endogenous levels of total c-Kit protein. It does not cross-react with other receptor tyrosine kinase family members. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7). |
| 存放说明 | -20C |
| 计算分子量 | 120 and 145 |
Western blot analysis of H526 cell lysate, using c-Kit (Ab81) Mouse mAb.用c-Kit (Ab81) Mouse mAb对H526细胞裂解液进行免疫印迹检测。 | |
Flow cytometric analysis of H526 cells using c-Kit (Ab81) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).用c-Kit (Ab81) Mouse mAb（蓝色）和非特异性阴性对照抗体（红色）对H526细胞进行流式细胞术检测。 | |
Confocal immunofluorescent analysis of NCI-H526 cells using c-Kit (Ab81) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用c-Kit (Ab81) Mouse mAb（绿色）对NCI-H526细胞进行免疫共聚焦检测。蓝色 = DRAQ5® #4084进行的DNA染色 |
