| 货号 | 2593S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Evi-1 (C50E12) Rabbit mAb detects the endogenous levels of total Evi-1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:400) IF-IC (1:1000) |
| 供应商 | CST |
| 背景 | Evi-1 (Ecotropic virus integration site 1) was originally identified as a common site of viral integration in murine myeloid leukemia. It is involved in human myeloid disorders through chromosome translocation and inversion (1) and is also implicated in solid tumor formation (2). Evi-1 is a zinc finger transcription factor which also plays an important role in animal development (3). It has many isoforms due to alternative usage of 5-ends (4), alternative splicing (5), and intergenic splicing which results in the formation of a fusion protein with MDS1 in normal tissues (6). |
| 存放说明 | -20C |
| 计算分子量 | 105, 145, 200 |
Confocal immunofluorescent analysis of SK-OV-3 (left) and DLD-1 cells (right) using Evi-1 (C50E12) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).共聚焦免疫荧光分析SK-OV-3细胞 (左图) 和DLD-1 细胞(右图)，所用抗体为Evi-1 (C50E12) Rabbit mAb (绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。 | |
Flow cytometric analysis of SK-OV-3 cells using Evi-1 (C50E12) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞仪分析SK-OV-3 细胞，所用抗体为Evi-1 (C50E12) Rabbit mAb (蓝色) 与非特异性对照抗体(红色)对比。 | |
Western blot analysis of extracts from various cell lines using Evi-1 (C50E12) Rabbit mAb. MDS/Evi-1 denotes the fusion between MDS1 and Evi-1 (see reference #6) and Evi-1 Δ denotes the truncated form of Evi-1 (see reference #5).Western免疫印迹。用 Evi-1 (C50E12) Rabbit mAb研究各种细胞系的细胞提取液。MDS/Evi-1表示MDS1和Evi-1 融合(见参考文献 6)，Evi-1 Δ 表示节删一部分的Evi-1 蛋白(见参考文献 5)。 |
