IKKα (3G12) Mouse mAb【科瑞奥博】

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IKKα (3G12) Mouse mAb

货号： 11930S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	11930S
反应种属	Human/Monkey
来源宿主	Mouse
应用	W/IF-IC/F
目标/特异性	IKKα (3G12) Mouse mAb recognizes endogenous levels of total IKKα protein.
使用方法	WB(1:1000) F (1:100) IF-IC (1:3200)

性能
供应商	CST
背景	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
存放说明	-20C
计算分子量	85

参考图片

Confocal immunofluorescent analysis of HCT 116 (high expression; left) and IGROV-1 (low expression; right) cells using IKKα (3G12) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦分析HCT116细胞（高表达；左图）和IGROV-1细胞（低表达；右图），使用抗体是IKKα (3G12) Mouse mAb （绿色）。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。

Western blot analysis of extracts from various cell lines using IKKα (3G12) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析多种细胞系的细胞提取物，使用抗体是 IKKα (3G12) Mouse mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。

Flow cytometric analysis of HCT 116 cells using IKKα (3G12) Mouse mAb (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red). Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.流失细胞术分析HCT116细胞的细胞提取物，使用抗体是 IKKα (3G12) Mouse mAb (蓝色)与 concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (红色)对应。 Anti-mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408用来作为二抗。