| 货号 | 3515S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:100) F (1:400) IF-IC (1:300) |
| 供应商 | CST |
| 背景 | Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-α), PMS2 (MutL-β) or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).错配修复(MMR)，一种涉及DNA合成过程中错误纠正的保守过程，对于维持基因组的完整性至关重要。MLH1是大肠杆菌MMR基因突变体的人类同源蛋白。MMR识别碱基错配或者插入/缺失回路需要MutL异源二聚体复合物招募而来的MutS同系物，该复合体由MLH1 和PMS1 (MutL-α), PMS2 (MutL-β)或者MLH3 (MutL-γ)组成。其它影响真核细胞中MMR的因子有EXO1, PCNA, RFC, RPA, DNA 聚合酶和DNA连接酶(回顾1)。MLH1基因失活可以引起基因组不稳定性和肿瘤易感性(2-5)。遗传性非息肉型结肠癌(HNPCC)中MLH1基因突变频繁(6)。MLH1也在减数分裂重组中起重要作用(7)。 |
| 存放说明 | -20C |
| 计算分子量 | 85 |
Western blot analysis of extracts from various cell types using MLH1 (4C9C7) Mouse mAb.western blot方法检测不同细胞提取物，使用的抗体为MLH1 (4C9C7) Mouse mAb。 | |
MLH1 was immunoprecipitated from HeLa cell lysates using MLH1 (4C9C7) Mouse mAb. Western blot was performed using the same antibody. Lane 1 is 5% input.从HeLa细胞裂解液中免疫沉淀MLH1，使用的抗体为MLH1 (4C9C7) Mouse mAb，随后用同一抗体进行western blot实验。泳道1为5%input。 | |
Confocal immunofluorescent analysis of HeLa cells using MLH1 (4C9C7) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光方法检测HeLa细胞，使用的抗体为MLH1 (4C9C7) Mouse mAb，呈绿色。肌动蛋白纤维使用DY-554 鬼笔环肽标记，呈红色。 | |
Flow cytometric analysis of IGROV-1 cells (red) and Jurkat cells (blue) using MLH1 (4C9C7) Mouse mAb.流式细胞术检测 IGROV-1细胞（红色）和Jurkat细胞（蓝色），使用的抗体为MLH1 (4C9C7) Mouse mAb。 |
