| 货号 | 11986S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | NTAL/LAB (D7I2B) Rabbit mAb recognizes endogenous levels of total NTAL/LAB protein. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Non-T cell activation linker (NTAL)/linker for activation of B cells (LAB) is a small transmembrane adaptor protein associated with glycolipid-enriched membrane fractions (1,2). NTAL/LAB is also known as LAT2 (linker for activation of T cells 2), WBSCR5, WBS15, and WBSCR15 (Williams-Beuren syndrome chromosome region 15 protein). It is expressed in B cells, monocytes, mast cells, and natural killer cells, but not in resting T cells (3). Upon activation of several receptors, NTAL/LAB becomes tyrosine-phosphorylated and recruits signaling molecules such as GRB2 and c-Cbl into receptor signaling complexes (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Confocal immunofluorescent analysis of SR (positive; left) and Jurkat (negative; right) cells using NTAL/LAB (D7I2B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦分析SR（阳性，左图）和Jurkat（阴性；右图）细胞，使用NTAL/LAB (D7I2B) Rabbit mAb (绿色)。 Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from RL-7, SEM, and Jurkat cells using NTAL/LAB (D7I2B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析RL-7、SEM、Jurkat细胞的细胞提取物，使用抗体是NTAL/LAB (D7I2B) Rabbit mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Human whole blood was fixed, lysed, and permeabilized as per the Cell Signaling Technology Flow Alternate Protocol and stained using NTAL/LAB (D7I2B) Rabbit mAb. Samples were co-stained with CD19-APC and CD3-PE to identify B and T cell populations, respectively. The forward/side scatter lymphocyte gate and CD19+ B cell population gate were combined and applied to a histogram depicting the mean fluorescence intensity of NTAL/LAB (blue) and a concentration-matched isotype control (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.收集人全血细胞，溶解，透析按照Cell S ignaling Technology公司交替流步骤进行，使用抗体是NTAL/LAB (D7I2B) Rabbit mAb。样品被CD19-APC和CD3-PE共处理，来鉴定B细胞核T细胞数。向前或侧向散射的淋巴细胞入口以及CD19+ B细胞数入口结合并应用到一个直方图的NTAL/LAB平均荧光强度(蓝色)中，相同浓度的对照（红色）。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412用来作为二抗。 |
