| 货号 | 15122S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/F |
| 使用方法 | WB(1:1000) F (1:50) |
| 供应商 | CST |
| 背景 | OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (left) or PHA-treated (1 μg/ml, 48 hr, 37ºC; right), using OX40 (D4N3K) Rabbit mAb and co-stained with anti-human CD4 antibody. Anti-rabbit IgG (H+L), F(ab)2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. | |
Western blot analysis of extracts from HuT 102, K-562, and Jurkat cells using OX40 (D4N3K) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human OX40 protein (hTNFRSF4), HVEM protein (hTNFRSF14), or GITR protein (hTNFRSF18), using OX40 (D4N3K) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
